Automation-friendly assays with "add-mix-measure" protocols to simplify and speed up your epigenetics research.
HDAC-Glo™ I/II Assays
Measure histone deacetylation activity in cells, extracts or from purified enzyme sources with these simple, “Add-Mix-Measure” luminescent assays and screening systems.
Single-reagent-addition, homogeneous, luminescent assays and screening systems that measure the relative activity of the NAD+-dependent histone deacetylase class III enzymes (sirtuins; SIRTs) from purified enzyme sources.
DUB-Glo™ Protease Assay
An easy assay for DUB, SENP or NEDP Activity. Measures activity of deubiquitinating (DUB), deSUMOylating (SENP) and deneddylating (NEDP) proteases.
An article describing the HDAC-Glo™ I/II and SIRT-Glo™ Assays. The assays can be multiplexed with viability assays to correlate deacetylase inhibition with cellular fate.
Here we describe efforts to define the utility of the HDAC-Glo™ Assay using structurally diverse HDAC inhibitors and various cell lines.