Selenomethionine Protein Labeling Using the Escherichia coli Strain KRX

With Monster Green® fluorescent protein as a model, we can achieve an average of 90% or greater selenomethionine (Se-Met) incorporation when the GFP is expressed in the E. coli strain KRX. Therefore, it is feasible to produce Se-Met-labeled protein samples for structural biology applications, such as X-ray crystallography, using the Single Step (KRX) Competent Cells.

Promega Notes 96, 24–26.