Promega Corporation

In Vitro HisTag Pull-Down Assay Using MagZ™ Particles


  • Share
  • Print
  • Email
  • Download PDF


Recently we developed a novel product, the MagZ™ Protein Purification System, for the purification of expressed his-tagged protein from rabbit reticulocyte lysate. This protein purification system has a unique advantage over nickel-based immobilized metal ion affinity chromatography (IMAC) systems in that it specifically purifies His-tagged proteins from rabbit reticulocyte lysate without co-purifying hemoglobin. This article demonstrates that MagZ™ Binding Particles can be used to develop in vitro His-tag pull-down assays. By using a His-tagged Id protein attached to MagZ™ Particles, we show that we can detect the interaction with MyoD.

Promega Notes 88, 13–15.

Becky Godat and Tonny Johnson

Promega Corporation
Publication Date: 2004

Download Article (164 KB)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Spring adventure!