T4 DNA Ligase
Joins Two DNA Strands with Cohesive or Blunt Ends
- Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors)
- Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
- Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
Catalog Number:
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Catalog Number: M1801
Catalog Number: M1804
Catalog Number: M1794
T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Reference
- Engler, M.J. and Richardson, C.C. (1982) In: The Enzymes, Boyer, P.D., ed., Academic Press, New York, NY.
Note: Units listed are Weiss units.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.
Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
T4 DNA Ligase 10X Buffer |
C126B | 1 × 200μl | View Product |
|
T4 DNA Ligase |
M180A | 1 × 100u |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
T4 DNA Ligase 10X Buffer |
C126A | 2 × 500μl | View Product |
|
T4 DNA Ligase |
M180B | 1 × 500u |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
T4 DNA Ligase 10X Buffer |
C126B | 1 × 200μl | View Product |
|
T4 DNA Ligase (HC) |
M179A | 1 × 500u |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
Resources
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