Cut Site:
CG AT▼CG
GC▲TA GC
Usage Notes:
- Religation of DNA fragments with dam methylation is difficult.
- Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.
Incubation Conditions: Buffer D. 37°C.
Source: Proteus vulgaris.
Storage Buffer: 10mM Tris-HCl (pH 7.3 @ 25°C), 50mM NaCl, 0.1% Triton® X-100, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.