HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack
- Available as a standalone product or as part of the HaloTag® Cloning Starter Kit (Cat.# G6050)
Catalog Number:
Size
Catalog Number: G3780
The vectors contained in the sample pack are configured to append the HaloTag® protein to either the carboxy- (pFC14K, pFC15K, pFC16K and pFC17K vectors) or amino-terminus (pFN21A, pFN21K, pFN22K, pFN23K and pFN24K vectors) of the protein fusion partner and provide constitutive protein expression in mammalian cells. The promoter in this vector series is based on the human cytomegalovirus (CMV) intermediate/early enhancer/promoter to support expression in mammalian cells at physiologically relevant levels. In some cell types, the vector series can provide four levels of expression, ranging from very high (full-length CMV) to very low (CMVd3, the most extreme deletion), spaced about 0.5 to 1 orders of magnitude apart.
Each vector contains the following features:
- A T7 RNA polymerase promoter for in vitro HaloTag® fusion protein expression. All vectors but pFC14K, pFN21K and pFN21A also have the SP6 RNA polymerase promoter for in vitro expression.
- A TEV protease site for cleavage of the expressed protein from HaloTag® using ProTEV Protease (Cat.# V6051).
- The lethal barnase gene for positive selection of the insert.
- Either an ampicillin-resistance gene (pFN21A) or kanamycin-resistance gene for selection of the plasmid.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pFC17K HaloTag® CMVd3 Flexi® Vector |
G132B | 1 × 2μg |
|
pFC16K HaloTag® CMVd2 Flexi® Vector |
G157B | 1 × 2μg |
|
pFC15K HaloTag® CMVd1 Flexi® Vector |
G160B | 1 × 2μg |
|
pFN21A HaloTag® CMV Flexi® Vector |
G282B | 1 × 2μg |
|
pFN21K HaloTag® CMV Flexi® Vector |
G283B | 1 × 2μg |
|
pFN22K HaloTag® CMVd1 Flexi® Vector |
G285B | 1 × 2μg |
|
pFN23K HaloTag® CMVd2 Flexi® Vector |
G287B | 1 × 2μg |
|
pFN24K HaloTag® CMVd3 Flexi® Vector |
G298B | 1 × 2μg |
|
pFC14K HaloTag® CMV Flexi® Vector |
G966B | 1 × 2μg |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,420,367, 8,748,148, 9,593,316 and 9,873,866; European Pat. Nos. 2374875, 2492342 and 2502990; and Japanese Pat. Nos. 5680302 and 5840117.
For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.
Resources
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