QuantiFluor® dsDNA System
Sensitive Quantitation of Double-Stranded DNA in Solution
- Specific quantitation of dsDNA
- More sensitive than NanoDrop for low-concentration samples
Catalog Number:
Size
Catalog Number: E2670
Catalog Number: E2671
Simple, Accurate DNA Quantitation
The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important. This dye shows minimal binding to single stranded DNA (ssDNA), RNA, protein and interfering compounds, allowing specific quantitation of dsDNA.
Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems
The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. 10pg/µl vs. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume).
| Sample* | Assay | |
|---|---|---|
|
QuantiFluor® dsDNA System
|
0.01–200ng/µl
|
0.01–200ng
|
|
Quant-iT™ PicoGreen
|
0.02–200ng/µl
|
0.02–200ng
|
|
Qubit® dsDNA HS
|
0.2–100ng/µl
|
0.2–100ng
|
|
NanoDrop® 2000 Spectrophotometer
|
2–12,000ng/µl
|
2–12,000ng
|
*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.
Better Sensitivity than Quant-iT™ PicoGreen®, Equivalent Linearity
The QuantiFluor® dsDNA System detects as little as 50pg/ml dsDNA in a 96-well microplate assay (1µl of a 10ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.
Proven for DNA Quantitation in Next-Gen Sequencing
Many laboratories have published papers using QuantiFluor® dyes in sequencing applications.
Easy Setup on Microplate or Single-Tube Fluorometers
System includes all the necessary reagents to quickly set up and quantitate dsDNA, and is easy to set up on microplate or single tube fluorometers, including the following Promega fluorometers.
How do fluorescent dyes compare to other quantitation methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
QuantiFluor® dsDNA Dye |
E258A | 1 × 1ml | |
|
Lambda DNA Standard |
E259A | 1 × 100μg | |
|
20X TE Buffer (pH 7.5) |
E260A | 1 × 25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
QuantiFluor® dsDNA Dye, 200X |
E258B | 1 × 100μl | 0.3mM | |
|
Lambda DNA Standard |
E259B | 1 × 10μg | ||
|
20X TE Buffer (pH 7.5) |
E260B | 1 × 5ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
Resources
Articles
- Evaluation of cfDNA as an early detection assay for dense tissue breast cancer
- Calibrating the Qubit® Fluorometer for QuantiFluor™ dsDNA System
- Calibrating the Qubit® 2.0 Fluorometer for use with the QuantiFluor™ dsDNA System
- Using the QuantiFluor™ dsDNA System and the GloMax® Multi+ Fluorometer with Instinct™ Software
Software and Firmware
Related Products
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