Wizard® SV 96 Plasmid DNA Purification System Technical Bulletin
Instructions for Use of Product(s)
A2250, A2255, A2291
Literature # TB272
The Wizard® SV 96 Plasmid DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA from as many as 96 samples. The entire miniprep procedure can be completed in 60 minutes or less. The purified plasmid can be used directly for automated fluorescent DNA sequencing as well as other standard molecular biology techniques, including restriction enzyme digestion.
Plasmid DNA is purified from bacterial lysates using a 96-well vacuum filtration step to simultaneously clear the bacterial lysate and bind plasmid DNA, eliminating the need for centrifugation. Washing of the bound plasmid DNA requires removal of only the Lysate Clearing Plate and Manifold Collar. Filtrate waste products are delivered directly to a vacuum trap, eliminating the need to empty waste collection flasks during DNA recovery. DNA is collected by eluting into a 96-well plate.
Summary of Changes
The following changes were made to the 2/26 revision of this document:
1. Deleted Note in Section 3.
2. Moved up Note and restored DNA Washing instructions in Section 3.A.
3. Inserted Step 4, Section 3.B, to instruct users on adding Cell Lysis Solution, and renumbered subsequent steps.
4. Added pressure measurements table by Section 3.B, Step 7.
5. Rearranged Table 2.
6. Made minor formatting changes.
Revised 2/26.