ADP-Glo™ Lipid Kinase Assay Technical Manual
Instructions for Use of Product(s)
V1721, V1731, V1741, V1751, V1761, V1771, V1781, V1782, V1791, V1792, V1690, VB3300, VB3310
Literature # TM365
ADP-Glo™ Lipid Kinase Systems provide a complete set of reagents for performing phosphoinositide lipid kinase (PIK) reactions using a luminescent ADP-detection platform, the ADP-Glo™ Kinase Assay. The systems include purified recombinant proteins of class I PI3Ks, optimized reaction buffer and ready-to-use lipid kinase substrates. The lipid kinase reaction is performed by incubating lipid substrate with a recombinant enzyme and ATP, and the kinase activity is measured using the ADP-Glo™ Kinase Assay. The ADP-Glo™ Kinase Assay is performed in two steps. First, after the kinase reaction, an ATP-depletion reagent is added to terminate the lipid kinase reaction and deplete any remaining ATP, leaving only ADP. Second, a detection reagent is added to simultaneously convert ADP to ATP and allow the newly synthesized ATP to be converted to light using a coupled luciferase/luciferin reaction.
Summary of Changes
The following changes were made to the 1/26 revision of this document:
1. Added Cat.# VB3300 and VB3310.
2. Removed Cat.# V1701 and V1711 as standalone products.
3. Edited Sections 1 and 2.
4. Removed Related Products and renumbered Summary of Changes.
5. Removed expired patent statement.
Revised 1/26.