IL-6 Bioassay Technical Manual
Instructions for Use of Product(s)
JA2501, JA2505, JA3501, JA3505
Literature # TM593
The IL-6 Bioassay is a bioluminescent cell-based assay designed to measure IL-6 stimulation or inhibition using a response element as a readout. The IL-6 Bioassay Cells are provided in a thaw-and-use format, as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation. The IL-6 Bioassay consists of human cells engineered to express the IL-6 receptor and a luciferase reporter driven by a response element (RE).When IL-6 binds, the IL-6R transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System and a standard luminometer, such as the GloMax® Discover System.
Summary of Changes
The following changes were made to the 5/26 revision of this document:
1. Removed Section 8, Related Products.
2. Made minor text and formatting edits.
Revised 5/26.