Enhanced Protein Mass Spectrometry Analysis with Trypsin/Lys-C Mix Scientific Poster

Part # PS181

Abstract

Marjeta Urh¹, Sergei Saveliev¹, Ethan Strauss¹, Mike Rosenblatt¹, Richard Jones², Michael Ford² and Dave Allen^2
1Promega Corporation, Madison, WI; ²MS BioWorks, Ann Arbor, MI

Efficient proteolysis is required for protein mass spectrometry analysis. Here we describe a Trypsin/Lys-C mix, which dramatically improves proteolysis:

• The majority of missed cleavages, commonly occurring in trypsin digests, are largely eliminated
• Protease-inhibiting impurities are tolerated
• Proteolytically resistant proteins are efficiently digested

We show here how enhanced proteolysis with Trypsin/Lys-C improves protein mass spectrometry analysis. These benefits include an increase in identified peptides and proteins, higher signal intensity of individual peptides and higher analytical reproducibility.

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