The Single-Use Cells are 50µl aliquots of chemically competent cells in single-use tubes. Transformation is performed directly in the provided tube, saving you time and effort and minimizing loss of efficiency due to repeated freeze-thaw cycles.
Single Step KRX Competent Cells
Efficient transformation and tightly controlled protein expression. Ideal for rapidly generating new clones for recombinant protein expression.
Single-Use Pro 5-alpha Competent Cells
High-efficiency competent cells (109cfu/µg), ideal for the blue/white screening of recombinant clones. Pro 5-alpha can be used for the efficient transformation of unmethylated DNA from PCR, cDNA and many other sources.
pGEM® 3Zf+/- Vectors
Multi-purpose, blue/white screening vector with many unique restriction sites. Use as a standard cloning vector, for in vitro transcription, or for production of circular ssDNA.
T4 DNA Ligase
Blue/White cloning qualified. Use to join two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either sticky- or blunt-end configuration.
The easy way to calculate melting temperature, molarity, dilution, DNA and protein concentration.
This teaching unit covers the basics of cloning and blue/white selection. Includes math-intensive lab